Premium
Polymer–protein interaction, water retention, and biocompatibility of a stimuli‐sensitive superporous hydrogel containing interpenetrating polymer networks
Author(s) -
Yin Lichen,
Zhao Ziming,
Hu Yizhe,
Ding Jieying,
Cui Fuying,
Tang Cui,
Yin Chunhua
Publication year - 2008
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.27744
Subject(s) - biocompatibility , interpenetrating polymer network , polymer , self healing hydrogels , polymer chemistry , swelling , ionic strength , chitosan , acrylamide , chemistry , acrylic acid , materials science , chemical engineering , copolymer , organic chemistry , composite material , aqueous solution , engineering
The swelling of a superporous hydrogel containing poly(acrylic acid‐ co ‐acrylamide)/ O ‐carboxymethyl chitosan interpenetrating polymer networks (SPH‐IPN) was sensitive toward the pH, ionic strength, and temperature stimuli. With insulin as a model drug, polymer–protein interaction was detected, and it was physical rather than covalent. Freezing water was the majority of the imbibed water in the swollen SPH‐IPNs, and the water‐retention ability of the polymer against compression and time of exposure at 37°C was improved as the amount of the O ‐carboxymethyl chitosan network increased. A 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) assay on AD293 and RBL‐2H3 cells and an in situ lactate dehydrogenase assay and morphological study on rat intestine confirmed that the SPH‐IPNs had satisfactory biocompatibility. These pronounced properties suggested that the SPH‐IPNs could be developed as an attractive peroral delivery vehicle for peptide and protein drugs. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008