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Studies of adsorption of alkaline trypsin by poly(methacrylic acid) brushes on chitosan membranes
Author(s) -
Bayramoğlu Gülay,
Erdogan Hakan,
Arica M. Yakup
Publication year - 2007
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.27690
Subject(s) - chitosan , membrane , adsorption , chemistry , polymer chemistry , methacrylic acid , epichlorohydrin , polyelectrolyte , poly(methacrylic acid) , aqueous solution , langmuir adsorption model , ionic strength , trypsin , ammonium persulfate , nuclear chemistry , chemical engineering , copolymer , organic chemistry , polymerization , polymer , biochemistry , engineering , enzyme
Poly(methacrylic acid)‐grafted chitosan membranes (chitosan‐ g ‐poly(MAA)) were prepared in two sequential steps: in the first step, chitosan membranes were prepared by phase‐inversion technique and then epichlorohydrin was used as crosslinking agent to increase its chemical stability in acidic media; in the second step, the graftcopolymerization of methacrylic acid onto the chitosan membranes was initiated by ammonium persulfate (APS) under nitrogen atmosphere. The chitosan‐ g ‐poly(MAA) membranes were first used as an ion‐exchange support for adsorption of trypsin from aqueous solution. The influence of pH, equilibrium time, ionic strength, and initial trypsin concentration on the adsorption capacity of the chitosan‐ g ‐poly(MAA) membranes have been investigated in a batch system. Maximum trypsin adsorption onto chitosan‐ g ‐poly(MAA) membrane was found to be 92.86 mg mL −1 at pH 7.0. The experimental equilibrium data obtained for trypsin adsorption onto chitosan‐ g ‐poly(MAA) membranes fitted well to the Langmuir isotherm model. The adsorption data was analyzed using the first‐ and second‐order kinetic models, and the experimental data was well described by the second‐order equation. More than 97% of the adsorbed trypsin was desorbed using glutamic acid solution (0.5 M , pH 4.0). In addition, the chitosan‐ g ‐ poly(MAA) membrane prepared in this work showed promising potential for various biotechnological applications. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2008