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Polycarbonate microspheres containing tumor necrosis factor‐α genes and magnetic powder as potential cancer therapeutics
Author(s) -
Hu Bin,
Yan GuoPing,
Zhuo RenXi,
Wu Yuan,
Fan ChangLie
Publication year - 2007
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.27544
Subject(s) - ethylene glycol , in vivo , polycarbonate , peg ratio , in vitro , tumor necrosis factor alpha , nuclear chemistry , materials science , hepatocellular carcinoma , cytotoxicity , chemistry , polymer chemistry , biochemistry , cancer research , biology , organic chemistry , immunology , microbiology and biotechnology , finance , economics
Amphiphilic polycarbonate copolymers including methoxy‐terminated poly(ethylene glycol)‐ co ‐poly (5,5‐dimethyl trimethylene carbonate) [Poly(PEG‐ b ‐TMC)] and poly(ethylene glycol)‐ co ‐poly(trimethylene carbonate) [Poly(PEG‐ b ‐DTC)] were synthesized. The water‐in‐oil‐in‐water (W/O/W) solvent evaporation technique was adopted to produce anticancer magnetic Poly(PEG‐ b ‐DTC) microspheres containing tumor necrosis factor‐α (TNF‐α) genes and Fe 3 O 4 magnetic ultrafine powder. Drug release studies showed that the microspheres can sustain a steady release rate of TNF‐α genes in 0.1 M phosphate buffer saline solution in vitro for up to 60 h. In vitro cytotoxicity assays demonstrated that the microspheres have high inhibition and antitumor action to human hepatocellular carcinoma (Bel‐7204) cells in vitro . In vivo inhibition on the growth of hepatic carcinomas and histopathologic observation indicated that the microspheres possess a markedly high antitumor activity to human hepatocellular carcinoma (Bel‐7204). © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2008