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Synthesis of ( Z )‐3‐hexen‐1‐yl acetate by lipase immobilized in polyvinyl alcohol nanofibers
Author(s) -
Nakane Koji,
Hotta Toshiki,
Ogihara Takashi,
Ogata Nobuo,
Yamaguchi Shinji
Publication year - 2007
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.26710
Subject(s) - lipase , nanofiber , polyvinyl alcohol , electrospinning , immobilized enzyme , acetic acid , materials science , chemistry , polymer chemistry , chemical engineering , nuclear chemistry , organic chemistry , polymer , enzyme , composite material , engineering
Abstract Polyvinyl alcohol (PVA)‐nanofibers‐immobilized lipase were formed by electrospinning. The specific surface area of the nanofiber (5.96 m 2 /g) was about 250 times larger than that of PVA‐film‐immobilized lipase (0.024 m 2 /g). The PVA‐nanofibers‐immobilized lipase were used as the catalyst for the esterification of ( Z )‐3‐hexen‐1‐ol (leaf alcohol) with acetic acid in hexane. The activity of the nanofiber is equivalent to that of commercially available immobilized lipase (Novozym‐435). The ester conversions of the nanofibers, Novozym‐435, the film and lipase powder reached 99.5% at 5 h, 100% at 5 h, 11.5% at 6 h, and 81.1% at 5.75 h, respectively. The nanofibers‐immobilized lipase showed higher activity for the esterification than the film‐immobilized lipase and lipase powder, probably because it has high specific surface area and high dispersion state of lipase molecules in PVA matrix. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007