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A novel potential cationic polymeric gene vector containing hydroxy groups and all grades of amino groups
Author(s) -
Wu Chuanbao,
Hao Jianyuan,
Deng Xianmo,
Liu Yu
Publication year - 2007
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.26372
Subject(s) - protonation , cationic polymerization , absorbance , polyethylenimine , dynamic light scattering , aqueous solution , polymer chemistry , titration , polyelectrolyte , fourier transform infrared spectroscopy , chemistry , polymer , zeta potential , nuclear chemistry , nanoparticle , materials science , chemical engineering , organic chemistry , chromatography , ion , nanotechnology , transfection , biochemistry , engineering , gene
Abstract A novel potential cationic polymeric gene vector, PCT, was synthesized by copolymerizing 3‐chloro‐1,2‐epoxypropane (CEP) and tetraethylene pentamine (TEPA). The protonizable nitrogen content of PCT is 17.7 mmol/g at 1 : 1 of CEP to TEPA, lower than 23.3 mmol/g of polyethylenimine (PEI) but higher than 7.8 mmol/g of polylysine, which indicates PCT is a candidate of polycation with high‐content but discrete charge after protonation. PCT has many hydrophilic quaternized amino and hydroxy groups contributing to hydrophilicity. Acid base titration showed that PCT was protonated in both pH 7.4–11 and pH 5.0–7.4 and had “proton sponge effect.” In aqueous solution, PCT complexed with weak anionic polymer, poly(acrylic acid) (PAA), into nanoparticles. Fourier transform infrared spectroscopy proved the formation of complex. Particle size is first increased and then decreased as increasing mass ratio θ of PCT to PAA from 0.1 to 1.7, as shown by dynamic light scattering. At θ values of more than 1.7 or less than 0.2, complex particles with mean diameter less than 200 nm were obtained. At θ of 0.5, the UV‐Vis absorbance of complex solution at first day was obviously higher than that at 7 day, while at θ of 1.7, two absorbance curves at these two time points had slightly smaller change, indicating that small complex particles were more stable than large ones because of electrostatic repulsion. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007