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Synthesis of ethyl propionate catalyzed by poly( N ‐AEAAm‐ co ‐AAc)‐ cl ‐MBAm hydrogel‐immobilized lipase of Bacillus coagulans MTCC‐6375
Author(s) -
Kanwar Shamsher Singh,
Kaushal Rajeev Kumar,
Aggarwal Amit,
Chauhan Sandeep,
Chimni Swapandeep Singh,
Chauhan Ghansham Singh
Publication year - 2007
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.26309
Subject(s) - propionate , lipase , bacillus coagulans , chemistry , nuclear chemistry , catalysis , organic chemistry , enzyme , fermentation
A purified alkaline thermotolerant bacterial lipase of Bacillus coagulans MTCC‐6375 was efficiently immobilized onto poly( N ‐AEAAm‐ co ‐AAc‐ cl ‐MBAm)‐hydrogel at pH 8.5 and at temperature 55°C in 16 h. The hydrogel‐bound matrix possessed 1.04 U/g (matrix) lipase activity with a specific activity of 1.8 U/mg of protein. The immobilized lipase resulted in formation of 52.5 m M of ethyl propionate (52% conversion) at 55°C in 9 h in n ‐nonane. Ethanol and propionic acid when used in a ratio of 300 : 100 m M , respectively, in n ‐nonane along with 10 mg of hydrogel‐bound lipase resulted in optimal synthesis of ethyl propionate (82.5 m M ). Addition of molecular sieves (3 Å, 0.7 g/reaction volume) further enhanced the conversion rate to 82.4% resulting in 83.5 m M of ethyl propionate. Incubation temperature below or above 55°C had a marked effect on the synthesis of ethyl propionate. However, esterification performed in n ‐heptane at 65°C resulted in 87.5 m M of ethyl propionate with a conversation rate of 89.3%. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007