Application of conducting poly(aniline‐ co ‐pyrrole) film to cholesterol biosensor | Zendy

Solanki Pratima R. | Zendy; Singh Suman | Zendy; Prabhakar Nirmal | Zendy; Pandey M. K. | Zendy; Malhotra B. D. | Zendy

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Application of conducting poly(aniline‐ co ‐pyrrole) film to cholesterol biosensor

Author(s) -

Solanki Pratima R.,

Singh Suman,

Prabhakar Nirmal,

Pandey M. K.,

Malhotra B. D.

Publication year - 2007

Publication title -

journal of applied polymer science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.575

H-Index - 166

eISSN - 1097-4628

pISSN - 0021-8995

DOI - 10.1002/app.26198

Subject(s) - cholesterol oxidase , glutaraldehyde , amperometry , fourier transform infrared spectroscopy , nuclear chemistry , biosensor , polymer chemistry , materials science , chemistry , analytical chemistry (journal) , chromatography , chemical engineering , organic chemistry , electrochemistry , electrode , nanotechnology , enzyme , engineering

Cholesterol oxidase (ChOx) has been covalently immobilized onto poly(aniline‐ co ‐pyrrole), electrochemically deposited onto indium‐tin‐oxide (ITO) glass plates, using glutaraldehyde as a crosslinker. These poly (An‐ co ‐Py)/ChOx films have been characterized using UV–visible spectroscopy fourier transform infrared spectroscopy, scanning electron microscopy, and photometric and amperometric techniques, respectively. The poly(An‐ co ‐Py)/ChOx bioelectrodes have been utilized for cholesterol estimation in the range of 1–10 m M . The ChOx activity in poly(An‐ co ‐Py)/ChOx bioelectrode has been found to be the highest at pH 7.0 at 25°C. The sensitivity and stability of poly(An‐ co ‐Py)/ChOx bioelectrode have been experimentally determined as 93.35 μA/m M and 10 weeks at 4°C, respectively. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2007

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