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Application of conducting poly(aniline‐ co ‐pyrrole) film to cholesterol biosensor
Author(s) -
Solanki Pratima R.,
Singh Suman,
Prabhakar Nirmal,
Pandey M. K.,
Malhotra B. D.
Publication year - 2007
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.26198
Subject(s) - cholesterol oxidase , glutaraldehyde , amperometry , fourier transform infrared spectroscopy , nuclear chemistry , biosensor , polymer chemistry , materials science , chemistry , analytical chemistry (journal) , chromatography , chemical engineering , organic chemistry , electrochemistry , electrode , nanotechnology , enzyme , engineering
Abstract Cholesterol oxidase (ChOx) has been covalently immobilized onto poly(aniline‐ co ‐pyrrole), electrochemically deposited onto indium‐tin‐oxide (ITO) glass plates, using glutaraldehyde as a crosslinker. These poly (An‐ co ‐Py)/ChOx films have been characterized using UV–visible spectroscopy fourier transform infrared spectroscopy, scanning electron microscopy, and photometric and amperometric techniques, respectively. The poly(An‐ co ‐Py)/ChOx bioelectrodes have been utilized for cholesterol estimation in the range of 1–10 m M . The ChOx activity in poly(An‐ co ‐Py)/ChOx bioelectrode has been found to be the highest at pH 7.0 at 25°C. The sensitivity and stability of poly(An‐ co ‐Py)/ChOx bioelectrode have been experimentally determined as 93.35 μA/m M and 10 weeks at 4°C, respectively. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2007