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Optimization of elution conditions of chitosan gel and the study of its adsorbent mechanism
Author(s) -
Tang ZhenXing,
Qian JunQing,
Shi LuE
Publication year - 2006
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.25327
Subject(s) - adsorption , chitosan , elution , freundlich equation , hydrogen bond , diffusion , chemistry , langmuir , chemical engineering , chromatography , materials science , thermodynamics , organic chemistry , molecule , physics , engineering
Chitosan gel was prepared by crosslinking method. To make use of chitosan gel, the optimization of elution conditions and the adsorbent characteristics of chitosan gel were discussed in this article. The optimum elution conditions were c (NaCl) = 0.05 mol/L in Tris‐HCl (pH, 9.05) at the flow rate of 2.0–3.0 mL/min with chitosan gel (particle sizes, 120–140 μm). The effects of contact time, pH, initial BSA concentration, and temperature on adsorption were studied. The equilibrium data could be described well by Langmuir, Freundlich, and Redlich–Peterson models. Adsorption dynamics had been successfully studied by Langergren, intraparticle diffusion model and Avrami model. The thermodynamics parameters Δ G °, Δ H °, and Δ S ° were calculated. The spectra studies indicated that the interaction between gel and protein was chiefly through electrostatic attraction in the shape of hydrogen bond. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 103: 1495–1506, 2007