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Antibody purification using porous metal–chelated monolithic columns
Author(s) -
Bereli Nilay,
Uzun Lokman,
Yavuz Handan,
Elkak Assem,
Denizli Adil
Publication year - 2006
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.23894
Subject(s) - monolith , adsorption , swelling , (hydroxyethyl)methacrylate , aqueous solution , methacrylate , desorption , materials science , polymerization , metal ions in aqueous solution , nuclear chemistry , polymer chemistry , chemical engineering , chemistry , chromatography , metal , polymer , catalysis , organic chemistry , composite material , metallurgy , engineering
Abstract A novel monolithic material was developed to obtain efficient and cost‐effective purification of IgG from human plasma. The porous monolith was obtained by bulk polymerization in a glass tube of 2‐hydroxyethyl methacrylate (HEMA) and N ‐methacryloyl‐( L )‐histidine methyl ester (MAH). The poly(HEMA‐MAH) monolith had a specific surface area of 214.6 m 2 /g and was characterized by swelling studies, porosity measurement, FTIR, scanning electron microscopy, and elemental analysis. Then the monolith was loaded with Cu 2+ ions to form the metal chelate. Poly(HEMA‐MAH) monolith with a swelling ratio of 74% and containing 20.9 μmol MAH/g was used in the adsorption/desorption of IgG from aqueous solutions and human plasma. The maximum adsorption of IgG from an aqueous solution in phosphate buffer was 10.8 mg/g at pH 7.0. Higher adsorption was obtained from human plasma (up to 104.2 mg/g), with a purity of 94.1%. It was observed that IgG could be repeatedly adsorbed and desorbed with the poly(HEMA‐MAH) monolith without significant loss of adsorption capacity. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 101: 395–404, 2006