Premium
Fractionation of a silk fibroin hydrolysate and its protective function of hydrogen peroxide toxicity
Author(s) -
Yeo JooHong,
Lee KwangGill,
Kweon HaeYong,
Woo SoonOk,
Han SangMi,
Kim SungSu,
Demura Makoto
Publication year - 2006
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.23740
Subject(s) - fibroin , bombyx mori , hydrolysate , silk , hydrogen peroxide , sericin , fractionation , bombycidae , chemistry , size exclusion chromatography , hydrolysis , polymer chemistry , phenylalanine , molecular mass , materials science , amino acid , biochemistry , organic chemistry , enzyme , composite material , gene
Fractionated components of Bombyx mori silk fibroin, which were hydrolyzed with protease, were prepared with a preparative‐recycling high‐performance‐liquid‐chromatography system to evaluate the protective effects of molecular‐weight‐controlled B. mori silk fibroin components on H 2 O 2 ‐injured neuronal cells. Three major fractions, having molecular weights less than about 1500, were first collected with the recycling techniques. The highest protective effect of the molecular‐weight‐controlled B. mori silk fibroin components on H 2 O 2 ‐injured neuronal cells was obtained when a fraction with a molecular weight of approximately 1400 was used. This protective effect of the silk fibroin hydrolysate on H 2 O 2 ‐injured neuronal cells was suggested to correlate with the contents of aromatic amino acids such as tyrosine and phenylalanine. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 102: 772–776, 2006