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Polysaccharide‐based artificial extracellular matrix: Preparation and characterization of three‐dimensional, macroporous chitosan and chondroitin sulfate composite scaffolds
Author(s) -
Peng ChihKang,
Yu ShuHuei,
Mi FwuLong,
Shyu ShinShing
Publication year - 2005
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.22730
Subject(s) - glutaraldehyde , chitosan , carbodiimide , biocompatibility , chondroitin sulfate , materials science , composite number , tissue engineering , biomaterial , nuclear chemistry , chemistry , polymer chemistry , nanotechnology , biomedical engineering , biochemistry , composite material , organic chemistry , glycosaminoglycan , medicine , metallurgy
Scaffold‐guided tissue engineering based on synthetic and natural occurring polymers has gained much interest in recent years. In this article, the development of a polysaccharide‐based artificial extracellular matrix (AECM) is reported. Three‐dimensional, macroporous composite AECMs composed of chondroitin sulfate (ChS) and chitosan (Chito) were prepared by an interpolyelectrolyte complex/lyophilization method. The ChS–Chito composite AECMs were crosslinked with glutaraldehyde and calcium ions (Ca 2+ ) and cocrosslinked with N,N ‐(3‐dimethylaminopropyl)‐ N ′‐ethyl carbodiimide (EDC) and N ‐hydroxysuccinimide (NHS). The crosslinking reactions were examined with Fourier transform infrared analysis. Glutaraldehyde and Ca 2+ crosslinked with Chito and ChS, respectively, to produce different types of ChS–Chito semi‐interpenetrated networks. In contrast, EDC/NHS crosslinked with both Chito and ChS to produce ChS–Chito connected networks. In physiological buffer solutions, the Ca 2+ ‐crosslinked ChS–Chito composite AECMs showed a lower swelling ratio than their EDC/NHS‐ and glutaraldehyde‐crosslinked counterparts. The ChS–Chito composite AECMs showed excellent antibacterial capability and biocompatibility according to the results of the in vitro antibacterial test and cytotoxic assay. This result suggested that the ChS–Chito composite AECMs might be a potential biomaterial for scaffold‐guided tissue‐engineering applications. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci, 2006