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Enzyme electrodes for determination of total phenolic capacity of red wines
Author(s) -
Kıralp S.,
Cirpan A.,
Toppare L.
Publication year - 2005
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.22165
Subject(s) - scanning electron microscope , electrode , pyrrole , tyrosinase , enzyme , copolymer , materials science , chemistry , immobilized enzyme , polymer chemistry , chemical engineering , polymer , organic chemistry , composite material , engineering
A tyrosinase‐modified electrode, based on physical entrapment of enzyme in copolymer matrix of decanedioic acid bis(4‐pyrrol‐1‐yl‐phenyl) ester (DAPE) with pyrrole was constructed and used to investigate the total amount of phenolics in red wines. Kinetic parameters, V max , and K m , were determined. Also, temperature, pH optimizations, operational stability and shelf life of the enzyme electrode were examined. Surface morphologies of the films were studied by scanning electron microscope. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 98: 521–524, 2005