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High‐performance liquid chromatography separation characteristics of molecular‐imprinted poly(methacrylic acid) microparticles prepared by suspension polymerization
Author(s) -
Kim Kayon,
Kim Dukjoon
Publication year - 2005
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.21419
Subject(s) - methacrylic acid , chromatography , high performance liquid chromatography , polymer , monomer , suspension polymerization , polymerization , suspension (topology) , particle size , dispersant , chemical engineering , materials science , molecular imprinting , molecularly imprinted polymer , polymer chemistry , chemistry , organic chemistry , selectivity , dispersion (optics) , catalysis , optics , homotopy , pure mathematics , engineering , physics , mathematics
Molecular‐imprinted poly(methacrylic acid) was synthesized with a template of retinoic acid to separate retinoid derivatives. The suspension polymerization technique was used to prepare round microparticles for high‐performance liquid chromatography (HPLC) packing column materials. The effects of the types and amounts of the dispersing agents and surfactants on the structure and size of the prepared molecular‐imprinted‐polymer particles were investigated. The separation of retinoic acid from its derivatives was more efficient when the perfluorocarbon dispersing agent was used instead of water, as the latter reduced the binding force between the objective molecules and monomers. HPLC separation features were also affected by the size and distribution of particles loaded in the column. A higher retention volume was obtained for smaller particles with a broader size distribution. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 96: 200–212, 2005