Premium
Cu(II)‐incorporated, histidine‐containing, magnetic‐metal‐complexing beads as specific sorbents for the metal chelate affinity of albumin
Author(s) -
Akgöl Sinan,
Türkmen Deniz,
Denizli Adil
Publication year - 2004
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.20757
Subject(s) - suspension polymerization , ethylene glycol dimethacrylate , adsorption , chemistry , nuclear chemistry , isoelectric point , ethylene glycol , desorption , glycidyl methacrylate , bovine serum albumin , polymer chemistry , polymerization , dispersion polymerization , chelation , chromatography , inorganic chemistry , organic chemistry , polymer , methacrylic acid , enzyme
N ‐Methacryloyl‐( L )‐histidine methyl ester (MAH) was synthesized from metharyloyl chloride and histidine. Spherical beads with an average size of 150–250 μm were obtained by the suspension polymerization of ethylene glycol dimethacrylate and MAH in an aqueous dispersion medium. Magnetic poly(ethylene glycol dimethacrylate‐ co ‐ N ‐Methacryloyl‐( L )‐histidine methyl ester) [m‐p(EGDMA‐ co ‐MAH)] microbeads were characterized with swelling tests, electron spin resonance, elemental analysis, and scanning electron microscopy. The specific surface area of the beads was 80.1 m 2 /g. m‐p(EGDMA‐ co ‐MAH) microbeads with a swelling ratio of 40.2% and 43.9 μmol of MAH/g were used for the adsorption of bovine serum albumin (BSA) in a batch system. The Cu(II) concentration was 4.1 μmol/g. The adsorption capacity of BSA on the Cu(II)‐incorporated beads was 19.2 mg of BSA/g. The BSA adsorption first increased with the BSA concentration and then reached a plateau, which was about 19.2 mg of BSA/g. The maximum adsorption was observed at pH 5.0, which was the isoelectric point of BSA. The BSA adsorption increased with decreasing temperature, and the maximum adsorption was achieved at 4°C. High desorption ratios (>90% of the adsorbed BSA) were achieved with 1.0 M NaSCN (pH 8.0) in 30 min. The nonspecific adsorption of BSA onto the m‐p(EGDMA‐ co ‐MAH) beads was negligible. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 93: 2669–2677, 2004