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Hemolglobin encapsulation in chitosan/calcium alginate beads
Author(s) -
Huguet M. L.,
Groboillot A.,
Neufeld R. J.,
Poncelet D.,
Dellacherie E.
Publication year - 1994
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1994.070510810
Subject(s) - chitosan , chemistry , sodium alginate , hemoglobin , chemical engineering , calcium alginate , calcium , chromatography , sodium , nuclear chemistry , organic chemistry , engineering
A mild chitosan/calcium alginate encapsulation process, as applied to encapsulation of hemoglobin, was investigated. The first procedure consisted of adding dropwise a hemoglobin‐containing sodium alginate mixture in a chitosan solution, then hardening the interior of capsules thus formed, in the presence of CaCl 2 . In the second method, the droplets were directly pulled off in a chitosan–CaCl 2 mixture. Both procedures led to beads containing a high concentration in entrapped hemoglobin as more than 90% of the initial concentration (150 g/L) were retained inside the beads provided that the chitosan concentration was great enough. The molecular weight of chitosan (M̄ u 245,000 or 390,000) and the pH of its solution (2, 4, or 5.4) had only a slight effect, the best retention being obtained with beads prepared at pH 5.4. The hemoglobin release during the bead storage in water was found to depend on the conditions of their formation and especially on the chitosan molecular weight. The best retention during storage in water was obtained with beads prepared with the high M̄ u chitosan solution at pH 2. Considering the total loss in hemoglobin during the bead formation and after 1 month of storage in water, the best results were obtained by preparing the beads in an 8 g/L solution of a 390,000 chitosan at pH 4 (less than 7% of loss with regard to the 150 mg/L initial concentration). © 1994 John Wiley & Sons, Inc.