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Synthesis of NAD polystyrene microspheres and application as a carrier of glucoamyrase immobilization
Author(s) -
Omi Shinzo,
Kaneko Kazuyoshi,
Takesue Mariko,
Tsujimura Hamana,
Satoh Atsunori,
Iso Mamoru
Publication year - 1994
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1994.070510711
Subject(s) - carbodiimide , polystyrene , emulsion polymerization , immobilized enzyme , polymerization , hydrolysis , polymer chemistry , chemistry , acrylic acid , yield (engineering) , dispersion polymerization , enzyme , chromatography , chemical engineering , materials science , copolymer , organic chemistry , polymer , metallurgy , engineering
Abstract Polystyrene microspheres with a small amount of acrylic acid (200 : 1) incorporated were synthesized by nonaqueous phase dispersion (NAD) polymerization to investigate the feasibility of use as a carrier for immobilized enzyme. Seeded emulsion polymerization was then carried out to obtain larger cross‐linked polystyrene spheres, the structure of which was expected to yield a favorable environment to keep the original activity of the immobilized enzyme. Glucoamylase, a particular enzyme catalyzing the hydrolysis of oligosaccharides to glucose, was immobilized through peptide bonding using a carbodiimide activating agent. Thirty percent of the enzyme activity at maximum was retained relative to free glucoamylase. The immobilized enzyme remained active after 4 months, although its poor resistance to heat was not improved. © 1994 John Wiley & Sons, Inc.