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Universal calibration assessment in aqueous gel permeation chromatography
Author(s) -
Bahary W. S.,
Jilani M.
Publication year - 1993
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1993.070480904
Subject(s) - calibration curve , polyacrylic acid , aqueous solution , chromatography , calibration , gel permeation chromatography , sodium polyacrylate , polymer , methanol , chemistry , materials science , analytical chemistry (journal) , permeation , phase (matter) , detection limit , organic chemistry , physics , membrane , raw material , quantum mechanics , biochemistry
It was the objective of this paper to assess the applicability of the universal calibration method to aqueous GPC/SEC with nonionic and anionic polymers using the Viscotek differential viscosity detector. Three water‐soluble polymers—polyacrylic acid, dextran, and polyethylene oxide—were chromatographed using four Ultrahydrogel TM columns with 0.3 M NaCl and 0.1 M KH 2 PO 4 as the mobile phase adjusted to pH 7. Three distinct calibration curves were obtained. Upon addition of 10% methanol, a reasonably good universal calibration curve was obtained. However, quantitative analysis of the data exhibited about 5% deviation in average M w and M n for sodium polyacrylate as calculated from the single curve as opposed to about 40% when calculated from the composite curve. The applicability of three theoretical models for the universal calibration method was assessed, and a recommendation was made for future work. © 1993 John Wiley & Sons, Inc.