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HPLC and 13 C‐NMR study of carboxymethyl‐β‐(1 → 6)‐ D ‐gluco‐β‐(1 → 3)‐ D ‐glucan derived from saccharomyces cerevisiae
Author(s) -
S̆oltés Ladislav,
Alföldi Juraj,
S̆andula Jozef
Publication year - 1993
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1993.070480801
Subject(s) - glucan , high performance liquid chromatography , chemistry , size exclusion chromatography , nuclear magnetic resonance spectroscopy , chromatography , yeast , saccharomyces cerevisiae , fraction (chemistry) , nmr spectra database , analytical chemistry (journal) , nuclear chemistry , stereochemistry , spectral line , organic chemistry , biochemistry , enzyme , physics , astronomy
Sodium salt of carboxymethyl‐β‐(1 → 6)‐D‐gluco‐β‐(1 → 3)‐D‐glucan (CMG‐Na) was prepared from β‐D‐glucan isolated from baker's yeast ( Saccharomyces cerevisiae ). Three samples, Fractions I, II, and III, were further separated from the crude CMG‐Na derivative. For the physicochemical characterization of the separated fractions, the methods of high‐performance liquid chromatography (HPLC) in the size‐exclusion mode and carbon−13 nuclear magnetic resonance ( 13 C‐NMR) spectroscopy were applied. The HPLC method revealed that the molecular weights, M n , M w , and M z averages, of Fraction II were 9.71 × 10 4 , 2.27 × 10 5 , and 3.59 × 10 5 Da, respectively, whereas those of Fraction III were 1.52 × 10 4 , 2.13 × 10 4 , and 3.57 × 10 4 Da, respectively. The 13 C‐NMR spectra of Fraction II showed a ratio of 3 : 1 for β−(1 → 3) / β−(1 → 6), whereas for Fraction III, the content of β‐(1 → 3) units was smaller. © 1993 John Wiley & Sons, Inc.