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Immobilization of glucose oxidase on nonwoven fabrics with bombyx mori silk fibroin gel
Author(s) -
Asakura Tetsuo,
Kitaguchi Motohiro,
Demura Makoto,
Sakai Harutoshi,
Komatsu Keiichi
Publication year - 1992
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1992.070460106
Subject(s) - fibroin , silk , viscose , bombyx mori , materials science , glucose oxidase , polymer chemistry , nonwoven fabric , sericin , chemical engineering , composite material , chemistry , fiber , nanotechnology , biosensor , biochemistry , engineering , gene
The enzyme glucose oxidase (GOD) was immobilized on the nonwoven fabrics, which have excellent properties in diffusivity of substrates, mechanical strength, and handling, with Bombyx mori silk fibroin gel. The nonwoven fabrics of silk fibroin, viscose rayon, poly‐ethyleneterephthalate, 6‐nylon, and polypropylene with activated surface by fluoline treatment were used. The stabilities of GOD to heat or pH changes were much improved by the immobilization as well as the case of the GOD immobilized in the silk fibroin membrane. Among nonwoven fabrics, silk fibroin was the most excellent support material for the immobilization of GOD although all nonwoven fabrics used here are able to be used as the support materials. The increase of the sensitivity was observed when the glucose sensor was made with the GOD immobilized on nonwoven silk fabrics as four times compared with the case of the GOD immobilized in the silk fibroin membrane.