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Size‐exclusion chromatography of high molecular weight water‐soluble polymers
Author(s) -
Safieddine Abbas M.,
Hester Roger D.
Publication year - 1991
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1991.070431104
Subject(s) - size exclusion chromatography , polymer , chromatography , gel permeation chromatography , elution , fractionation , aqueous solution , molar mass distribution , resolution (logic) , chemistry , column chromatography , materials science , organic chemistry , artificial intelligence , computer science , enzyme
A preparative‐scale, aqueous size‐exclusion chromatography system was constructed for fractionation of large molecular weight polymers. Calibration of molecular weight to elution volume was accomplished without polymer standards by using an eluent viscosity detector in series with a refractometer. The system was found to have a hydrodynamic size‐separation resolution that ranged from 500 to 3500 Å.