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Immobilization of papain on crosslinked polymer supports: Role of the macromolecular matrix on enzymic activity
Author(s) -
Jayakumari V. G.,
Pillai V. N. Rajasekharan
Publication year - 1991
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1991.070420303
Subject(s) - papain , polystyrene , macromolecule , polymer , immobilized enzyme , polyethylene glycol , polymer chemistry , chemistry , polyacrylamide , enzyme assay , enzyme , chemical engineering , materials science , organic chemistry , biochemistry , engineering
Papain was immobilized on polymer supports with spacer arms of varying nature and length. As the length of the spacer arm increased, there was a marked increase in the extent of enzyme immobilization and activity of immobilized enzymes. When a long, flexible and hydrophilic polyethylene glycol spacer was introduced between the polystyrene backbone and the functional group used for immobilization, the extent of coupling and enzyme activity increased. Dependence of enzyme activity on the nature and extent of crosslinking and on the nature of the polymeric backbone was investigated. Hydrophilic polyacrylamide‐based supports were found to be more efficient supports for immobilization compared to hydrophobic polystyrene‐based supports.