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Effective immobilization of protein linked with polyethylene glycol on silica via hydrogels using silica sol
Author(s) -
Yoshinaga Kohji,
Kito Taketoshi,
Yamaye Makoto
Publication year - 1990
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1990.070410707
Subject(s) - peg ratio , polyethylene glycol , bovine serum albumin , reagent , polymer chemistry , silylation , self healing hydrogels , chemistry , materials science , composite number , methanol , chemical engineering , nuclear chemistry , organic chemistry , chromatography , catalysis , composite material , finance , engineering , economics
An effective attachment of activated polyethylene glycol (PEG), which has dichloro‐ s ‐triazinyl functional groups, to silica and protein immobilization with the resulting PEG/SiO 2 composite were studied. The reaction of methanol—silica sol with the PEG—silyl reagent resulted in an efficient PEG attachment to SiO 2 as compared with the usual two‐step PEG modification of silica gel and glass surface. The activated PEG/SiO 2 was able to immobilize over 600 mg of bovine serum albumin per unit gram of the PEG composite through hydrogel formation. The application of the present binding method of PEG‐linked protein on SiO 2 was also discussed.