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Limitation and evaluation of the two‐broad standard (TBS) method of calibration of aqueous SEC
Author(s) -
Omorodion S. N. E.,
Hamielec A. E.
Publication year - 1990
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1990.070390409
Subject(s) - calibration curve , calibration , analytical chemistry (journal) , function (biology) , dispersion (optics) , materials science , chromatography , chemistry , mathematics , optics , physics , statistics , evolutionary biology , detection limit , biology
Few years ago, the two broad standard method of molecular weight (MW) calibration was proposed 1 and the presence of negative σ 2 (peak dispersion coefficient) was found to be somewhat disturbing. The limitation of the method was not specified. Since large negative values of σ 2 cannot be tolerated, herein is reported another type of instrumental spreading function for which the method may seem to apply, and an evaluation of this method. In its evaluation, plots of log e (intercept of a linear molecular weight calibration curve), that is, log e ( D 1 ) vs. the corresponding slope of the molecular weight calibration curve, D 2 , which were found to be linear, were used. The systems employed were Dextran/Corning controlled porous glass (CPG‐10) packing in well‐chosen mobile phase.