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Sample overloading effects in polymer characterization by field‐flow fractionation
Author(s) -
Caldwell Karin D.,
Brimhall Steven L.,
Gao Yushu,
Giddings J. Calvin
Publication year - 1988
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1988.070360319
Subject(s) - thermal diffusivity , field flow fractionation , polymer , fractionation , materials science , analytical chemistry (journal) , characterization (materials science) , viscosity , sample (material) , flow (mathematics) , thermal , chemistry , chromatography , composite material , thermodynamics , mechanics , physics , nanotechnology
There are two subtechniques of field‐flow fractionation (FFF), thermal FFF and flow FFF, that have been successfully employed for polymer fractionation and characterization. These techniques are primarily analytical in nature, yielding accurate polymer characteristics from small sample loads (∼ 10 μg or less, depending on detection sensitivity). In this study the effects of increasing sample size are examined. Modest increases in load are found to result in shifts toward higher retention volumes. These modest loads also result in some broadening of the sample peaks without a major loss of peak symmetry. Excessive loading, by contrast, appears to give rise both to skewed peaks and to new artifact peaks at higher levels of retention. These observations are discussed in terms of the concentration dependence of various properties (viscosity, diffusivity, thermal diffusivity) which influence polymer transport through the FFF channel. The results are used to indicate upper limits to suitable sample concentrations.