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Pretreatment of eucalyptus wood with sodium hypochlorite and enzymatic hydrolysis by cellulases of Trichoderma viride
Author(s) -
David C.,
Fornasier R.,
Lejong W.,
Vanlautem N.
Publication year - 1988
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1988.070360103
Subject(s) - trichoderma viride , cellulase , chemistry , enzymatic hydrolysis , cellulose , hydrolysis , sodium hypochlorite , factorial experiment , substrate (aquarium) , nuclear chemistry , chromatography , food science , organic chemistry , biology , ecology , statistics , mathematics
Eucalyptus saligna meal and chips have been treated with HClO‐NaClO in order to increase their accessibility to cellulases from Trichoderma viride and hence to increase the yield of hydrolysis of cellulose into glucose. Different experimental variables determine the efficiency of the pretreatment. These are the pH and temperature of the pretreatment, the granulometry and concentration of the substrate, the oxidant concentration and prior elimination of hemicelluloses by prehydrolysis with dilute acid. An incomplete factorial design has been used to study the effect of these experimental variables on the weight loss during the pretreatment, the composition of the pretreated substrate, and the quantity of glucose formed as function of time in the subsequent enzymatic hydrolysis. Delignification can reach 70% using 5 mol HClO‐NaClO per kilogram of wood.