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Characterization of nonderivatized cellulose by gel permeation chromatography
Author(s) -
Schwald Wolfgang,
Bobleter Ortwin
Publication year - 1988
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1988.070350719
Subject(s) - cellulose , gel permeation chromatography , chromatography , chemistry , column chromatography , permeation , solvent , materials science , organic chemistry , polymer , membrane , biochemistry
The development of a gel permeation chromatography (GPC) system for the analysis of nonderivatized cellulose in the DP range of technical cellulose products is reported. In contrast to traditional GPC, where cellulose derivatives have to be prepared, in this work the cellulose solvent cadoxen was applied for the determination of the molecular weight distribution using a special analysis system. Nuclear magnetic resonance studies of cadoxen solutions indicated no complex formation between cadoxen and cellulose. Therefore, to avoid precipitation of cellulose from the prepared solution, cadoxen was also used as eluent in the chromatographic system. For this reason a stationary phase had to be found which is stable under the given strong alkaline conditions. The utilization of Fractogel TSK as column material resulted in the separation of dissolved cellulose showing a DP v between 400 and 2000 with good reproducibility. The application of very sensitive detectors together with a GPC program allows the characterization of unknown cellulose samples of different origin within a few hours.