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Quantitative branching of linear and branched polysaccharide mixtures by size exclusion chromatography and on‐line low‐angle laser light scattering detection
Author(s) -
Yu LiPing,
Rollings J. E.
Publication year - 1988
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1988.070350420
Subject(s) - branching (polymer chemistry) , size exclusion chromatography , molar mass distribution , analytical chemistry (journal) , light scattering , chemistry , chromatography , multiangle light scattering , scattering , polymer , gel permeation chromatography , optics , organic chemistry , physics , enzyme
The quantitative branching characterization of polysaccharide via size exclusion chromatography (SEC) and on‐line low‐angle laser light scattering (LALLS) detection is presented from both theoretical arguments as well as direct experimental evidence. The two measurable branching parameters g v ( m ) [= ( M l /( M n ) m ) v ] and g ′ v ( m ) [ = ( M l /( M w ) m ) v ] of a sample mixture have been related theoretically to the mixture's composition. There exists linear relationships between g v ( m ) and W b,v (the mass fraction of branched component in mixture) as well as between g ′ v ( m ) −1 and w b,v . The latter correlation has been demonstrated experimentally employing a combined SEC/LALLS technique, and displays excellent agreement with the theoretical predictions. Furthermore, this polymer branching characterization method has been applied to study enzymatic starch hydrolysates products. The molecular weight distribution and branching distribution have been obtained.