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High performance size exclusion chromatography of starch with dimethyl sulfoxide as the mobile phase
Author(s) -
Chuang JauYi,
Sydor Robert J.
Publication year - 1987
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1987.070340431
Subject(s) - dimethyl sulfoxide , size exclusion chromatography , starch , chromatography , phase (matter) , chemistry , high performance liquid chromatography , sodium nitrate , organic chemistry , enzyme
Two types of high performance size exclusion chromatographic (SEC) columns were tested for the characterization of starch samples: μ Bondagel and Aquapore. The mobile phase was dimethyl sulfoxide (DMSO) containing 0.03 M sodium nitrate maintained at 80°C. The results indicated that both μ Bondagel and Aquapore can be used to determine the relative molecular weight of starch. The exclusion limits for these columns exceed ten million. Preliminary results using μ Styragel are also reported. Examples of SEC analysis of starch, including starch with anionic groups, are given.