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Osmocentrifugation in density gradients: Application to polymer latex separation
Author(s) -
WinklerHechenleitner Ana A.,
Costa M. Célia P.,
Galembeck F.
Publication year - 1987
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1987.070340234
Subject(s) - percoll , polymer , ficoll , centrifugation , materials science , chromatography , chemical engineering , chemistry , composite material , peripheral blood mononuclear cell , biochemistry , engineering , in vitro
Polymer latexes can be fractionated by size and by chemical composition through osmocentrifugation. Density gradients are formed by centrifuging Ficoll and Percoll solutions in dialysis cells; mixed polymer latex zones are layered over these gradients and centrifuged again, leading to separated latex bands from which polymer may be collected and further analyzed. Zone migration rates in the osmocentrifugation experiment are faster than in standard centrifugation tubes.