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Preparation of new hydrophilic poly‐vinylpyridine beads and their application to immobilization of urease
Author(s) -
Sugii A.,
Ogawa N.,
Matsumoto M.
Publication year - 1986
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1986.070320515
Subject(s) - urease , adsorption , polymer , urea , chemistry , ethylene glycol , ethylene glycol dimethacrylate , copolymer , polymer chemistry , immobilized enzyme , chromatography , enzyme , organic chemistry , methacrylic acid
Macroporous hydrophilic poly‐vinylpyridine beads were prepared by suspension copolymerization of 4‐ (or 2‐)vinylpyridine and hydrophilic cross‐linking agents, such as ethylene glycol dimethacrylate (1EG) and tetraethylene glycol dimethacrylate (4EG), and their physical properties were studied. Immobilized enzymes were obtained by adsorption of urease on vinylpyridine polymers and commercially available hydrophobic polymers. The poly‐4‐vinylpyridine resin crosslinked with 4EG (degree of crosslinkage, 25%) showed higher water regain, larger average pore radius, and the highest adsorption of urease. Leakage of urease from immobilized urease was scarcely observed in the buffer solution used. The hydrophilicity and hydrophobicity of support affected markedly the enzymatic reaction of the immobilized enzyme. As one of the applications of the polymer‐supported urease, blood urea nitrogen in human sera was determined.