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Effect of pretreatment of cellulosic wastes on the production of cellulase enzymes by Trichoderma reesei
Author(s) -
Kassim E. A.,
Ghazi I. M.,
Nagieb Z. A.
Publication year - 1986
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1986.070310306
Subject(s) - bagasse , cellulase , trichoderma reesei , sawdust , cellulose , chemistry , fermentation , cellulosic ethanol , hemicellulose , lignin , chloroform , food science , nuclear chemistry , pulp and paper industry , organic chemistry , engineering
The cellulosic wastes, bagasse and sawdust, were treated with different acidified solvents: chloroform, dioxan, ethanol, ethylacetate, dimethyl sulfoxide and dimethyl formamide; for increasing periods up to 36 h. The treated materials were delignified, dried and introduced in the fermentation media as inducing carbon source for Trichoderma reesei . The untreated materials and pure cellulose powder were used individually in control and reference media, respectively. The fermentation media containing bagasse treated for 8 h in chloroform or dioxan gave a promising cellulase activity, nearly the same as that from the reference medium. The untreated bagasse gave a markedly low cellulase activity. The media with treated sawdust gave a relatively higher enzyme activity but far below the reference medium. The sawdust treatment with the solvents mentioned resulted in a very mild effect because of its high content of admixtures, lighin and hemicellulose bonding cellulose.