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Studies on cellulase from trichoderma reesei and its effect on pretreated cellulosic materials
Author(s) -
Nagieb Z. A.,
Ghazi I. M.,
Kassim E. A.
Publication year - 1985
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1985.070301212
Subject(s) - cellulase , bagasse , trichoderma reesei , cellulosic ethanol , sawdust , chemistry , hydrolysis , enzymatic hydrolysis , nuclear chemistry , cellulose , pulp and paper industry , food science , organic chemistry , engineering
The stability of locally produced cellulase from T. reesei was studied. The enzyme solution was stable at temperatures up to 45°C over which a gradual inactivation occured until 55–60°C. A rapid loss of enzymatic activity was observed over 60°C and complete inactivation occurred at 70°C after only 1 h. The maximum enzymatic saccharification was obtained at 50°C and pH 6.5. The pretreatment of cellulosic wastes, bagasse, and sawdust from soft and hard wood was carried out in different solvents, chloroform, dioxan, dichloroethane, ethylacetate, and ethanol, for 8 h. These treatments increased the susceptibility of the cellulosic wastes to enzymatic saccharification. The bagasse was highly affected, followed by the sawdust from soft and hard wood, respectively.