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Immobilization of α‐amylase on polymeric carriers having different structures
Author(s) -
Ohtsuka Yasuji,
Kawaguchi Haruma,
Yamamoto Takuya
Publication year - 1984
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1984.070291108
Subject(s) - reagent , amylase , immobilized enzyme , chemistry , enzyme , texture (cosmology) , chemical engineering , materials science , polymer chemistry , chromatography , organic chemistry , artificial intelligence , computer science , engineering , image (mathematics)
α‐Amylase was immobilized onto several polymeric carriers having carboxyl groups by the Woodward's reagent K better than by other methods. The amount of immobilized α‐amylase mainly depended on the surface area of carriers, while the enzymic activity depended on the texture of carrier surface. Flat surface was favorable for making the enzyme exhibit high activity and for keeping it active in wide pH range. The durability of immobilized enzyme was excellent at 20°C and pH 5.5.