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Size exclusion chromatography of polyelectrolytes in dimethylformamide
Author(s) -
Scheuing D. R.
Publication year - 1984
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1984.070290912
Subject(s) - polyelectrolyte , polystyrene , styrene , methacrylic acid , polymer , dimethylformamide , sulfonate , chemistry , polymer chemistry , monomer , size exclusion chromatography , sodium bromide , chromatography , copolymer , sodium , organic chemistry , solvent , enzyme
A study of the size exclusion chromatographic behavior of styrene–methacrylic acid and epoxyacrylic polymers has been made. These polymers exhibit strong “ion‐exclusion” effects in DMF, similar to that found for water‐soluble polyelectrolytes such as sodium polystyrene sulfonate. The addition of lithium bromide, at a concentration of 0.1M, overcomes ion exclusion on a set of silicabased, “deactivated” duPont SEC columns. Calibration of both the duPont columns and a set of styrene–divinyl benzene based columns (μ‐Styragel) is complicated by absorption of polystyrene standards in DMF and DMF/salt mobile phases. The absorption of monomeric solutes on the duPont columns in DMF/salt mobile phases is different from that of their corresponding polymers.