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Immobilization of cellulase using porous polymer matrix
Author(s) -
Kumakura Minoru,
Kaetsu Isao
Publication year - 1984
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1984.070290903
Subject(s) - polymer , monomer , cellulase , porosity , matrix (chemical analysis) , polymerization , materials science , chemical engineering , immobilized enzyme , polymer chemistry , chemistry , cellulose , enzyme , organic chemistry , composite material , engineering
A new method is discussed for the immobilization of cellulase using porous polymer matrices, which were obtained by radiation polymerization of hydrophilic monomers. In this method, the immobilized enzyme matrix was prepared by enzyme absorbtion in the porous polymer matrix and drying treatment. The enzyme activity of the immobilized enzyme matrix varied with monomer concentration, cooling rate of the monomer solution, and hydrophilicity of the polymer matrix, taking the change of the nature of the porous structure in the polymer matrix. The leakage of the enzymes from the polymer matrix was not observed in the repeated batch enzyme reactions.