Premium
Preparation of photoresponsive polymeric adsorbent containing amphiphilic polymer with azobenzene moiety and its application for cell adhesion chromatography
Author(s) -
Ishihara Kazuhiko,
Kim Munsok,
Shinohara Isao,
Okano Teruo,
Kataoka Kazunori,
Sakurai Yasuhisa
Publication year - 1983
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1983.070280406
Subject(s) - azobenzene , moiety , adsorption , polymer , polymer chemistry , adhesion , amphiphile , materials science , chemical engineering , chemistry , copolymer , organic chemistry , engineering
In order to develop a new type column chromatography, a polymeric adsorbent was prepared by grafting a photoresponsive polymer containing an azobenzene moiety on the surface of controlled pore glass beads. The polarity of the surface of the polymeric adsorbent was increased by UV irradiation because of trans–cis isomerization of azobenzene moiety. Adhesion chromatography of erythrocytes was carried out using the photoresponsive polymeric adsorbent. The erythrocytes were adhered to the adsorbent in the dark and separated from the adsorbent by UV irradiation. This behavior appears to be caused by the photoresponse of the polarity change on the surface of the polymeric adsorbent. The morphology of the erythrocytes which adhered on the surface of the photoresponsive polymeric adsorbent remained unchanged both in the dark and after UV irradiation. The amount of erythrocytes which adhered to the adsorbent increased with increasing the molecular weight of the grafted polymer and the hydrophobicity of the surface.