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Differential gel permeation chromatography for quality control
Author(s) -
Runyon J. R.
Publication year - 1983
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1983.070280211
Subject(s) - gel permeation chromatography , polymer , chromatography , elution , calibration , differential (mechanical device) , permeation , column chromatography , calibration curve , materials science , analytical chemistry (journal) , chemistry , mathematics , membrane , statistics , detection limit , physics , thermodynamics , composite material , biochemistry
For the direct comparison of similar polymers, as, for example, for quality control, differential gel permeation chromatography provides a simple, sensitive technique that is relatively insensitive to operational variables. One polymer is chosen as a standard and a solution of that polymer is used as the eluent in an otherwise conventional GPC. Differential chromatograms of slightly different polymers are both positive and negative with respect to the baseline. Positive portions represent an excess and negative portions a deficiency as compared to the standard. As in conventional GPC, the elution volumes of the differences indicate the molecular size ranges of the differences. The algebraic sum of the differences is zero. Examination of the raw curves with only general information about calibration and operating conditions tells nearly as much about the differences as carefully executed conventional GPC with complex data reduction.