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Gel permeation chromatography: The effect of flow rate on efficiency. II
Author(s) -
Chuang JauYi,
Johnson Julian F.,
Cooper Anthony R.
Publication year - 1983
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1983.070280204
Subject(s) - volumetric flow rate , permeation , gel permeation chromatography , flow (mathematics) , chromatography , materials science , analytical chemistry (journal) , chemistry , mechanics , composite material , membrane , polymer , physics , biochemistry
The effect of flow rate on efficiency in gel permeation chromatoraphy has been examined over wide ranges of flow rate, i.e., linear flow velocities using columns of different diameters using both organic and inorganic packings. The results indicate a considerable increase in efficiency at flow velocities below 0.02 cm/s. No lower limit to this increase could be determined due to experimental difficulties. Between flow velocities of 0.02–0.2 cm/s little efficiency is lost.