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System development for size exclusion chromatography of starch hydrolysates
Author(s) -
Rollings J. E.,
Okos M. R.,
Tsao G. T.,
Bose A.
Publication year - 1982
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1982.070270636
Subject(s) - chromatography , size exclusion chromatography , particle size , aqueous solution , sodium hydroxide , ionic strength , chemistry , starch , calibration curve , polystyrene , materials science , column chromatography , fractionation , polymer , detection limit , organic chemistry , enzyme
A system for the aqueous size exclusion chromatography (SEC) of starch hydrolysates has been developed. Sepharose CL is the chromatographic support material, and aqueous sodium hydroxide solution is the eluent. Various factors affecting the resolution of the proposed system are discussed. Support materials having small particle size and narrow particle size distribution are necessary for maximizing separation efficiency. The ionic strength of the eluent, however, has a negligible effect on separation efficiency. The fractionation range of the system has been broadened by connecting in series columns containing two different Sepharose‐CL gel types according to the bimodal pore size distribution concept. Well‐characterized sodium polystyrene sulfonate and dextran standards are used to calibrate this SEC system. The Coll–Prusinowski calibration procedure leads to a linear calibration curve over a wide molecular weight domain.