Premium
Fibrous support for immobilization of enzymes
Author(s) -
Ichijo Hisao,
Suehiro Tetsuro,
Yamauchi Aizo,
Ogawa Shigeo,
Sakurai Masanori,
Fujii Nobuo
Publication year - 1982
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1982.070270523
Subject(s) - invertase , vinyl alcohol , enzyme , adsorption , fiber , immobilized enzyme , chemistry , polymer chemistry , chemical engineering , hydrolysis , biochemistry , organic chemistry , polymer , engineering
A poly(vinyl alcohol) (PVA) fiber support incorporating various aminoacetal functional groups has been developed for immobilizing enzymes. The aminated PVA fiber seems to adsorb enzymes with electrostatic force of attraction; thus the immobilization procedure is simple. By the use of this fiber having immobilized enzymes, the reaction between enzymes and substrates is nearly independent of the size of subtstrates. This newly developed type of fiber, which is formed by a mass superfine fibers (SFF), each measuring 1 μm or less in diameter, permits much more increased surface area than the conventional enzyme immobilization supports. Our studies of the properties of the fiber for immobilization of enzymes show the following results: (1) SFF has a greater ability for the immobilization of invertase than ordinary fibers; (2) dimethyl‐aminated SFF has the best performance for the immobilization of invertase. From these results, it is concluded that the dimethyl‐aminated SFF is an excellent support for the immobilization of invertase.