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Gel permeation chromatography using controlled‐porosity glass. I. Polyacrylamide–formamide solution
Author(s) -
Onda Nobuhiko,
Furusawa Kunio,
Yamaguchi Noriko,
Komuro Shūgi
Publication year - 1979
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1979.070231218
Subject(s) - polyacrylamide , formamide , gel permeation chromatography , chromatography , column chromatography , elution , hydrolysis , porosity , chemistry , permeation , phase (matter) , salt (chemistry) , high performance liquid chromatography , materials science , polymer chemistry , organic chemistry , polymer , membrane , biochemistry
The process of characterizing polyacrylamide and its partially hydrolyzed materials by gel permeation chromatography was examined. The use of controlled‐porosity glass and formamide as the stationary phase and the eluent, respectively, resulted in chromatographic behavior in accord with the hydrodynamic volume concept for polyacrylamide fractions. The addition of a salt (KCl) to the eluent was found to retard the elution of the hydrolyzed polyacrylamide.