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Use of sorbyl chloride–osmium staining for fibrillar measurements in cotton fibers
Author(s) -
Hebert J. J.,
Hensarling T. P.,
Jacks T. J.,
Berni R. J.
Publication year - 1979
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1979.070231010
Subject(s) - osmium tetroxide , osmium , staining , cellulose , moiety , chemistry , chloride , polymer chemistry , electron micrographs , negative stain , materials science , electron microscope , organic chemistry , pathology , medicine , physics , ruthenium , optics , catalysis
The technique for staining unsaturated additives within cotton fibers reacts osmium tetroxide with a sorbyl moiety which has been attached to the cellulose chain. Resulting electron micrographs indicate that contrast is considerably enhanced. Measurements of fibrillar size averaged 0.30 nm, closely approximating the values in the literature.