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Contribution of adsorption and partition to the separation mechanism in gel chromatography on inorganic carriers
Author(s) -
Bakoš D.,
Bleha T.,
Ozimá A.,
Berek D.
Publication year - 1979
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1979.070230802
Subject(s) - adsorption , elution , chromatography , polystyrene , partition coefficient , gel permeation chromatography , chemistry , volume (thermodynamics) , solubility , partition (number theory) , analytical chemistry (journal) , thermodynamics , polymer , organic chemistry , physics , mathematics , combinatorics
Gel permeation chromatography (GPC) calibration curves log hydrodynamic volume versus elution volumes were obtained from a series of single and mixed eluents for polystyrene on inorganic carriers. The observed calibration shifts were interpreted as a result of adsorption and partition effects on elution volume V e . Secondary contributions to the separation mechanism can be qualitatively described by means of thermodynamic relations from liquid adsorption (LSC) and liquid partition (LLC) chromatography using the solubility parameters of the eluents. The universal calibration procedure based on the hydrodynamic volume of the coils is directly applicable only for systems in which adsorption and partition is approximately the same.