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Comments on data treatment in gel permeation chromatography
Author(s) -
Ogawa Toshio,
Inaba Tadami
Publication year - 1976
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1976.070200807
Subject(s) - calibration curve , molar mass distribution , gel permeation chromatography , calibration , logarithm , chromatography , distribution (mathematics) , polymer , elution , curve fitting , analytical chemistry (journal) , chemistry , standard deviation , range (aeronautics) , materials science , mathematics , statistics , detection limit , mathematical analysis , organic chemistry , composite material
The effects of variables on treatment of the gel permeation chromatogram are reported. Variables investigated include (1) the molecular weight distribution of polymers for preparing the calibration curve, i.e., the logarithm molecular weight–elution count relationship, (2) nonlinearity of the calibration curve, and (3) fluctuation of the baseline. The deviation of the calibration curve prepared by the polymer having broad molecular weight distribution was evaluated in detail by assuming log‐normal distribution function for the distribution. The polymer having a D value less than 1.3 was recommended for this purpose. Generally, the shape of the chromatogram is fairly different from that of the true molecular weight distribution curve when the calibration curve is not linear over the entire range of interest. By fitting polynomials to the calibration curve, the chromatogram was sufficiently converted to the molecular weight distribution curve. The apparent difference between them was removed. Slight deviation of the baseline from the true one gave rise to obvious error in the calculated molecular weight and its distribution, especially for the sample having a broad distribution.