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Method for correcting molecular weight from gel‐permeation chromatography. I. Skewing and concentration corrections
Author(s) -
Nakano Seikou,
Goto Yukitaka
Publication year - 1975
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1975.070191002
Subject(s) - dispersity , gel permeation chromatography , molar mass distribution , polymer , chromatography , resolution (logic) , elution , volume (thermodynamics) , binary number , analytical chemistry (journal) , size exclusion chromatography , chemistry , sample (material) , sample preparation , materials science , polymer chemistry , mathematics , thermodynamics , computer science , organic chemistry , physics , arithmetic , artificial intelligence , enzyme
The purpose of this paper is to present a metod to calculate real molecular weight averages of polymer samples from GPC chromatograms where the instrumental spreading functions are skewed and the concentration effect exists. In this method, it is assumed that (1) the skewed chromatograms of monodisperse polymer samples can be represented as resultant of halves of two different Gaussians, (2) the resolution factors are regarded as constant in the case of low sample loading, and (3) the peak elution volume is independent of the presence of other components in the case of low sample loading. Adequate monodisperse polystyrenes and the mixtures (binary, seven and ten components) were examined for this purpose; and the molecular weight averages calculated by this method were compared with the ones obtained by Rosen and Provder's method. From the results in our study, it is found that this method can be available for correcting molecular weight from GPC chromatograms except for very narrow high molecular weight samples.