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Exclusion chromatography using controlled‐porosity glass. I. Comparison with styrene gels
Author(s) -
Spatorico A. L.
Publication year - 1975
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1975.070190611
Subject(s) - polystyrene , gel permeation chromatography , materials science , porosity , size exclusion chromatography , porous glass , chromatography , volume (thermodynamics) , polyester , methyl methacrylate , dispersion (optics) , analytical chemistry (journal) , monomer , polymer , composite material , chemistry , organic chemistry , physics , quantum mechanics , optics , enzyme
The instrumental parameters influencing the calibration of a gel permeation chromatograph (GPC) using column packings of Corning CPG‐10 porous glass have been evaluated. The porous glass system used a set of columns 1.6 × greated in length and 0.6 × smaller in diameter than the Styragel system, which was used for comparison. The dependence of retention volume of polystyrene calibrants on eluent flow rate and on sample size was similar in both systems. Samples of poly(methyl methacrylate), poly(vinyl choloride), and poly(4,4′‐isopropylidenediphenylene 1,1,3‐trimethyl‐3‐phenylindan‐4′,5‐dicarboxylate) fractions were examined, and the molecular weights, obtained by using the hydrodynamic volume concept, were in satisfactory agreement with the light‐scattering data for both chromatographic systems. Corrections for axial dispersion, for the polyester fractions and polystyrene samples, were probably equivalent in these two chromatographic systems.