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Improved calibration procedure for gel permeation chromatography of lignins
Author(s) -
Obiaga T. I.,
Wayman Morris
Publication year - 1974
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1974.070180704
Subject(s) - gel permeation chromatography , calibration curve , chromatography , molar mass distribution , chemistry , lignin , calibration , ultracentrifuge , molecular mass , permeation , diffusion , analytical chemistry (journal) , polymer , organic chemistry , membrane , thermodynamics , mathematics , physics , biochemistry , statistics , detection limit , enzyme
Abstract In studying macromolecular properties of lignins, gel permeation chromatography (GPC) is a convenient method for the determination of lignin molecular weights and their distribution. A GPC analytical column was calibrated by preparing narrow molecular weight lignin fractions, then applying the method of Goring and co‐workers to obtain molecular weights from the ultracentrifuge at zero concentration and zero field force. Diffusion and other curve‐broadening effects in the GPC analytical column were taken into account by applying the correction method of Hamielec and co‐workers to derive the coefficients of the calibration curve. The molecular weight of a lignosulfonate sample determined on the GPC was found to coincide closely with that obtained by ultracentrifugation.