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Gel permeation chromatography: The effect of treatment with hexamethyldisilazane on porous glass packings
Author(s) -
Cooper Anthony R.,
Johnson Julian F.
Publication year - 1969
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1969.070130711
Subject(s) - gel permeation chromatography , polystyrene , permeation , chromatography , porous glass , elution , porosity , polymer , solvent , fractionation , chemistry , materials science , substrate (aquarium) , chemical engineering , organic chemistry , membrane , biochemistry , engineering , oceanography , geology
Porous glass column packings have been shown to be an effective substrate for gel permeation chromatography. When carbon tetrachloride is used as a solvent in conjuction with infrared detection, the porous glass exhibits adsorptive properties with polystyrene and polyisobutene. A procedure to eliminate these undesirable properties by treatment with hexamethyldisilazane has been developed. Experiments showed complete elution of all injected polymer. The treatment thus permits quantitative fractionation studies using infrared detection.