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Solubilization of calfskin collagen in citrate buffer with the use of automated equipment
Author(s) -
Kahn Leo D.,
Witnauer Lee P.
Publication year - 1969
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1969.070130115
Subject(s) - buffer (optical fiber) , solubilization , ionic strength , electrolyte , phosphate buffered saline , chemistry , buffer solution , monomer , chromatography , solubility , chemical engineering , materials science , polymer , organic chemistry , aqueous solution , computer science , biochemistry , telecommunications , electrode , engineering
An automated collagen solubilizing apparatus that maintains constant electrolytic conditions during solubilization is described. It was used in solubilization of 6.95% of a calfskin corium as monomeric collagen in citrate buffer at pH 3.44 and ionic strength of 0.44 and a temperature of 8°C. It is shown that this is the limit of solubilization for undenatured collagen in this buffer.