Solubilization of calfskin collagen in citrate buffer with the use of automated equipment | Zendy

Kahn Leo D. | Zendy; Witnauer Lee P. | Zendy

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Solubilization of calfskin collagen in citrate buffer with the use of automated equipment

Author(s) -

Kahn Leo D.,

Witnauer Lee P.

Publication year - 1969

Publication title -

journal of applied polymer science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.575

H-Index - 166

eISSN - 1097-4628

pISSN - 0021-8995

DOI - 10.1002/app.1969.070130115

Subject(s) - buffer (optical fiber) , solubilization , ionic strength , electrolyte , phosphate buffered saline , chemistry , buffer solution , monomer , chromatography , solubility , chemical engineering , materials science , polymer , organic chemistry , aqueous solution , computer science , biochemistry , telecommunications , electrode , engineering

An automated collagen solubilizing apparatus that maintains constant electrolytic conditions during solubilization is described. It was used in solubilization of 6.95% of a calfskin corium as monomeric collagen in citrate buffer at pH 3.44 and ionic strength of 0.44 and a temperature of 8°C. It is shown that this is the limit of solubilization for undenatured collagen in this buffer.

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