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Entrap‐immobilization of invertase on composite gel fiber of cellulose acetate and zirconium alkoxide by sol–gel process
Author(s) -
Nakane Koji,
Ogihara Takashi,
Ogata Nobuo,
Kurokawa Youichi
Publication year - 2001
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.1642
Subject(s) - invertase , zirconium , fiber , hydrolysis , chemistry , cellulose , nuclear chemistry , immobilized enzyme , composite number , cellulose acetate , chromatography , polymer chemistry , sucrose , materials science , chemical engineering , inorganic chemistry , organic chemistry , composite material , enzyme , engineering
We prepared a composite gel fiber by the gel formation of cellulose acetate and zirconium tetra‐ n ‐butoxide. Gel fiber is stable in common solvents, phosphate solution, and electrolyte solution. Invertase was entrap‐immobilized on the gel fiber. The immobilization was easily performed under the mild conditions. The apparent Michaelis constant ( K m ) and maximum reaction velocity ( V max ) were estimated from Eadie–Hofstee plot for immobilized invertase. The K m of immobilized invertase was larger than that of native invertase, while the opposite tendency was observed for the V max . The activity for the immobilized invertase became higher with increasing fiber diameter. It indicates that the hydrolysis of sucrose occurs in the neighborhood of the fiber surface. The thermal stability of the immobilized invertase was higher than those of its native counterpart. © 2001 John Wiley & Sons, Inc. J Appl Polym Sci 81: 2084–2088, 2001